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Anti-Fibrillarin antibody - Nucleolar Marker
Anti-Fibrillarin antibody - Nucleolar Marker from Abcam
Be the first to write a review!
Citations:
(177)
Supplier Page
Supplier Page from
Abcam for
Anti-Fibrillarin antibody - Nucleolar Marker
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Fibrillarin antibody - Nucleolar Marker
Company
Abcam
Price
Pricing Info
Supplier Page
View Supplier Product Page
Catalog Number
ab5821
Antibody Type
Primary
Quantity
100 UG
Isotype
IgG
Clonality
Polyclonal
Applications
Immunocytochemistry/ Immunofluorescence, Western blot
Host
Rabbit
Conjugate/Tag
Unconjugated
Concentration
0.5 mg/ml
Research Area
Epigenetics
Reactivity
Drosophila melanogaster, Human, Mouse
Target
Fibrillarin
Gene Name
fbl
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (177)
(21)
Excess ribosomal protein production unbalances translation in a model of Fragile X Syndrome
Nature communications
June 10, 2022
Seo SS, Louros SR, Anstey N, Gonzalez-Lozano MA, Harper CB, Verity NC, Dando O, Thomson SR, Darnell JC, Kind PC,
et al
.
WT pairs using anti-Y10b (
Abcam
,
ab171119
) and anti-
NeuN
(
Merck
,
ABN78
) or anti-Fibrillarin (
Abcam
,
ab5821
) and anti-
NeuN
(
Millipore
,
MAB377
) antibodies, diluted according to the manufacturer’s specifications.
(22)
RNA is required for the integrity of multiple nuclear and cytoplasmic membrane-less RNP granules
The EMBO journal
May 2, 2022
Decker CJ, Burke JM, Mulvaney PK, Parker R.
A/C (
Cell Signaling
4777
)
1:500
,
mouse
anti‐PML (
Abcam
ab96051
)
1:500
, rabbit anti‐fibrillarin (
Abcam
ab5821
)
1:500
,
mouse
anti‐
SMN1
(
Cell Signaling
12976S
)
1:400
, goat anti‐rabbit IgG AF647 (
Abcam
ab150079
)
1:1,000
,
(23)
Erebosis, a new cell death mechanism during homeostatic turnover of gut enterocytes
PLoS biology
April 1, 2022
Ciesielski HM, Nishida H, Takano T, Fukuhara A, Otani T, Ikegawa Y, Okada M, Nishimura T, Furuse M, Yoo SK.
06–570
), rabbit-anti-cleaved-
Drosophila
Dcp-1
(
1:100
,
Cell Signaling
, #
9578
), rabbit-anti-Fibrillarin antibody (
1:100
,
Abcam
, #
ab5821
), rabbit-anti-beta Tubulin antibody (
1:100
,
Abcam
, #
ab179513
), Alexa Fluor phalloidin (
1:100
, A22287), Alexa
(24)
MicroRNA-101-3p Suppresses Cancer Cell Growth by Inhibiting the USP47-Induced Deubiquitination of RPL11
Cancers
February 15, 2022
Park J, Cho M, Cho J, Kim EE, Song EJ.
Probes, Eugene, OR, USA) for 1 h at room temperature. The nucleolus was stained with anti-fibrillarin (
ab5821
,
Abcam
), and DNA was detected by DAPI (Sigma-Aldrich). The
fluorescence
response HA-RPL11 and fibrillarin
(25)
Selective translation of epigenetic modifiers affects the temporal pattern and differentiation of neural stem cells
Nature communications
January 25, 2022
Wu Q, Shichino Y, Abe T, Suetsugu T, Omori A, Kiyonari H, Iwasaki S, Matsuzaki F.
been conjugated with eFluor660. The primary antibodies were: fibrillarin (
1:1000
, rabbit polyclonal,
ab5821
,
Abcam
), Cleaved
Caspase-3
(Asp175)(
1:400
, rabbit polyclonal,
9661S
,
Cell Signaling Technology
), Satb2
(26)
Hybridization-proximity labeling reveals spatially ordered interactions of nuclear RNA compartments
Molecular Cell
January 20, 2022
Karen Yap, Tek Hong Chung, Eugene V. Makeyev
fixation/permeabilization strategies depending on the antibody. For antibodies against fibrillarin (
Abcam
, cat#
ab5821
; RRID:AB_2105785;
1:500
dilution), hnRNPL (
Abcam
, cat#
ab32680
; RRID:AB_941986;
1:500
dilution) or MCM5
(27)
Systematic mapping of rRNA 2’-O methylation during frog development and involvement of the methyltransferase Fibrillarin in eye and craniofacial development in Xenopus laevis
PLOS Genetics
January 18, 2022
Jonathan Delhermite, Lionel Tafforeau, Sunny Sharma, Virginie Marchand, Ludivine Wacheul, Ruben Lattuca, Simon Desiderio, Yuri Motorin, Eric Bellefroid, Denis L. J. Lafontaine
membranes
and probed in TBS/0.1% tween /3% BSA. Probing was as follows: anti-fibrillarin (
Abcam
, Ref.
ab5821
) incubated overnight at
1:2000
dilution, followed by anti-rabbit HRP (Cytiva, Ref. NA934V) used at a
(28)
Alteration of ribosome function upon 5-fluorouracil treatment favors cancer cell drug-tolerance
Nature communications
January 10, 2022
Therizols G, Bash-Imam Z, Panthu B, Machon C, Vincent A, Ripoll J, Nait-Slimane S, Chalabi-Dchar M, Gaucherot A, Garcia M,
et al
.
PBS. Fibrillarin, Dyskerin and Nucleolin were detected using the anti-FBL rabbit polyclonal antibody (
ab5821
,
Abcam
) diluted at
1:2,000
, anti-DKC1 rabbit polyclonal (
sc-48794
,
Santa Cruz Biotechnology
) diluted
(29)
R-loops trigger the release of cytoplasmic ssDNAs leading to chronic inflammation upon DNA damage
Science advances
November 19, 2021
Chatzidoukaki O, Stratigi K, Goulielmaki E, Niotis G, Akalestou-Clocher A, Gkirtzimanaki K, Zafeiropoulos A, Altmüller J, Topalis P, Garinis GA.
WB: 1/500), GAPDH (glyceraldehyde phosphate dehydrogenase) (
ab8245
; WB: 1/4000), fibrilarin (FBL) (
ab5821
; WB: 1/2500), and β-tubulin (
ab6046
; WB: 1/1000) were from
Abcam
; cleaved
caspase 3
(#
9661
; IF: 1/50,
(30)
RNA promotes the formation of spatial compartments in the nucleus
Cell
November 11, 2021
Quinodoz SA, Jachowicz JW, Bhat P, Ollikainen N, Banerjee AK, Goronzy IN, Blanco MR, Chovanec P, Chow A, Markaki Y,
et al
.
RRID:AB_2722569); anti-
NPM1
(
Abcam
Cat#
ab10530
, RRID:AB_297271;
1:200
); anti-Fibrillarin (
Abcam
Cat#
ab5821
, RRID:AB_2105785;
1:200
); anti-LaminB1 (
Abcam
Cat#
ab16048
, RRID:AB_10107828;
1:1000
); For imaging studies,
(31)
SDE2 is an essential gene required for ribosome biogenesis and the regulation of alternative splicing
Nucleic acids research
September 20, 2021
Floro J, Dai A, Metzger A, Mora-Martin A, Ganem NJ, Cifuentes D, Wu CS, Dalal J, Lyons SM, Labadorf A,
et al
.
Phospho-
4E-BP1
-Thr37/46 (
Cell Signaling Technology
2855S
),
Cactin
(
Bethyl Laboratories
A303-349A
), FBL (
Abcam
ab5821
), Tubulin (
Cell Signaling Technology
2125S
), p54 (
Santa Cruz Biotechnology
sc-126
) and
p21
(
Santa Cruz Biotechnology
(32)
Targeting the methyltransferase SETD8 impairs tumor cell survival and overcomes drug resistance independently of p53 status in multiple myeloma
Clinical epigenetics
September 16, 2021
Herviou L, Ovejero S, Izard F, Karmous-Gadacha O, Gourzones C, Bellanger C, De Smedt E, Ma A, Vincent L, Cartron G,
et al
.
min, and saturated with PBS-1% BSA for 20 min. Anti-nucleolin (
ab22758
,
Abcam
) and anti-fibrillarin (
ab5821
,
Abcam
) antibodies were diluted 1/200 in saturation buffer, and incubated for 90 min in a humid chamber
(33)
The exon-junction complex helicase eIF4A3 controls cell fate via coordinated regulation of ribosome biogenesis and translational output
Science advances
August 1, 2021
Kanellis DC, Espinoza JA, Zisi A, Sakkas E, Bartkova J, Katsori AM, Boström J, Dyrskjøt L, Broholm H, Altun M,
et al
.
(
Sigma-Aldrich
,
05-636
),
mouse
monoclonal
UBF
(
Santa Cruz Biotechnology
,
sc-13125
), rabbit polyclonal FBL (
Abcam
,
ab5821
), rabbit polyclonal
RBM8A
(
Y14
) (
Thermo Fisher Scientific
,
PA5-53790
),
mouse
monoclonal nucleophosmin
(34)
Interactome Analysis of KIN (Kin17) Shows New Functions of This Protein
Current issues in molecular biology
July 22, 2021
Gaspar VP, Ramos AC, Cloutier P, Pattaro Junior JR, Duarte Junior FF, Bouchard A, Seixas FAV, Coulombe B, Fernandez MA.
was diluted
1:4000
and incubated for 60 min. As a nucleolar marker, Anti-Fibrillarin antibody (
Abcam
AB5821
) was used
1:500
, incubated for 60 min, and detected by Alexa Fluor 488-conjugated
donkey
anti-rabbit
(35)
Chromatin tracing and multiplexed imaging of nucleome architectures (MINA) and RNAs in single mammalian cells and tissue
Nature Protocols
May 1, 2021
Miao Liu, Bing Yang, Mengwei Hu, Jonathan S. D. Radda, Yanbo Chen, Shengyan Jin, Yubao Cheng, Siyuan Wang
56-40-6)
Yeast
tRNA (
Invitrogen
, cat. no.
15401011
) Anti-fibrillarin primary antibody (
Abcam
Cat#
ab5821
, RRID:AB_2105785) Alexa Fluor 647-conjugated anti-rabbit secondary antibody (
Thermo Fisher Scientific
(36)
Systemic and local effect of the Drosophila headcase gene and its role in stress protection of Adult Progenitor Cells
PLOS Genetics
February 8, 2021
Panagiotis Giannios, Jordi Casanova
#
9578
] (
1:100
); anti-phospho-4EBP [
Cell Signaling
, (Thr37/46), #
2855
] (
1:100
); anti-Fibrillarin [
Abcam
,
ab5821
] (
1:500
); anti-phospho-RpS6, kindly provided by A. Teleman [24] (
1:200
); anti-phospho-
p38
[
Cell Signaling
,
(37)
Multiplexed imaging of nucleome architectures in single cells of mammalian tissue
Nature Communications
December 1, 2020
Miao Liu, Yanfang Lu, Bing Yang, Yanbo Chen, Jonathan S. D. Radda, Mengwei Hu, Samuel G. Katz, Siyuan Wang
temperature for 30 min. The sample was then incubated with anti-fibrillarin primary antibody (
Abcam
Cat#
ab5821
) at a concentration of
1:100
in blocking buffer with 100× diluted murine RNase inhibitor at 4 °C overnight
(38)
Alpha-satellite RNA transcripts are repressed by centromere-nucleolus associations
eLife
November 11, 2020
Bury L, Moodie B, Ly J, McKay LS, Miga KH, Cheeseman IM.
auto-immune serum; Antibodies, Inc):
1:1000
, CENP-A (
Abcam
,
ab13939
) at
1:1000
, Fibrillarin (
Abcam
,
ab5821
) at
1:300
, and
Ki67
(
Abcam
,
ab15580
) at
1:100
. For smFISH, cells were fixed again for 10 min with 4%
(39)
Repression of interrupted and intact rDNA by the SUMO pathway in
Drosophila melanogaster
eLife
November 9, 2020
Luo Y, Fefelova E, Ninova M, Chen YA, Aravin AA.
Immunofluorescence microscopy
was performed as previously described (Hur et al., 2016). AntiFibrillarin antibody (
Abcam
ab5821
) was added to fixed ovaries at
1:500
dilution and incubated at 4˚ C overnight followed by
incubation
(40)
Reorganization of the nuclear architecture in the
Drosophila melanogaster
Lamin B mutant lacking the CaaX box
Nucleus
September 22, 2020
Semen M. Bondarenko, Igor V. Sharakhov
antibody ADL67.10 or ADL84.12 (DSHB, Iowa City, IA, USA), anti-fibrillarin primary rabbit antibody
ab5821
(
Abcam
, San Francisco, CA, USA),
anti-Lamin C
primary
mouse
antibody LC28.26 (DSHB, Iowa City, IA, USA),
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Anti-Fibrillarin antibody - Nucleolar Marker from Abcam
Supplier Page
View Supplier Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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